Composite

Part:BBa_K1162014:Design

Designed by: Ryan Putman   Group: iGEM13_Utah_State   (2013-09-17)


protmoter+rbs+N-terminal 10x His-Tag+GFP with no translational stop codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 269
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 788
    Illegal XhoI site found at 689
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RFC 23. ATG(Met) at start on coding region.


Source

GFP is synthetic form of GFP from a Jellyfish (Aequorea victoria).

References